On October 9, 2024, positive equine infectious anemia (EIA) results were confirmed by the Canadian Food Inspection Agency’s (CFIA) national reference laboratory for one mare located on a premises in Yellowhead 94 County, Alberta. A foal had initially been tested by an accredited veterinarian to fulfill an export requirement. The results required follow-up testing to be performed by the CFIA on both the foal and dam, and the dam was confirmed positive. Neither animal was displaying clinical signs at the time of sampling. A CFIA investigation is underway, and movement controls have been placed on the infected horse and any on-premises contact animals. Initial reports indicate there are several other equines on the affected premises. Movement controls will remain until all disease response activities have been completed, including follow-up testing and ordering the destruction of confirmed cases. Trace-out activities may require the CFIA to undertake actions at additional premises as outlined in the current policy. Improved biosecurity protocols have been strongly recommended to the owners to help control the ongoing spread of EIA and protect our national herd.